Automated dual capillary electrophoresis system with hydrodynamic injection for the concurrent determination of cations and anions
The capillary electrophoresis instrument developed for the concurrent
determination of cations and anions features two separate capillaries
and individual detectors to allow independent optimization for each
group of ions. The capillaries are joined in a common injector block.
The sample is drawn into the injector with a small membrane pump and
automated simultaneous injection into both capillaries is achieved by
pressurization of the fluid with compressed air. Flushing of the
injector and of the capillaries with the background electrolyte is also
carried out automatically by the same means. The buffer consisted of
12mM histidine and 2mM 18-crown-6 adjusted to pH 4 with acetic acid and
was suitable for the contactless conductivity detection employed. The
system was optimized for the determination of cationic NH4 + and anionic
NO3- and NO2-, and linear calibration curves from about 20μM up to
about 1.5mM were obtained for these ions. In a test run over 8h, the
reproducibility for the peak areas was within ±7%. For demonstration,
the instrument was successfully applied to the concurrent monitoring of
the concentrations of the three ions during the biological removal of
ammonium from contaminated groundwater in a sequencing batch reactor,
where NO3- and NO2- are formed as intermediate products
Title:
Title:
| Automated dual capillary electrophoresis system with hydrodynamic injection for the concurrent determination of cations and anions | |
| Authors: | Pham, Thi Thanh Thuy Mai, Thanh Duc Nguyen, Thanh Dam Sáiz, Jorge |
| Keywords: | Anions Dual-capillary electrophoresis Simultaneous separations |
| Issue Date: | 2014 |
| Publisher: | Analytica Chimica Acta |
| Citation: | Scopus |
| Abstract: | The capillary electrophoresis instrument developed for the concurrent determination of cations and anions features two separate capillaries and individual detectors to allow independent optimization for each group of ions. The capillaries are joined in a common injector block. The sample is drawn into the injector with a small membrane pump and automated simultaneous injection into both capillaries is achieved by pressurization of the fluid with compressed air. Flushing of the injector and of the capillaries with the background electrolyte is also carried out automatically by the same means. The buffer consisted of 12mM histidine and 2mM 18-crown-6 adjusted to pH 4 with acetic acid and was suitable for the contactless conductivity detection employed. The system was optimized for the determination of cationic NH4 + and anionic NO3- and NO2-, and linear calibration curves from about 20μM up to about 1.5mM were obtained for these ions. In a test run over 8h, the reproducibility for the peak areas was within ±7%. For demonstration, the instrument was successfully applied to the concurrent monitoring of the concentrations of the three ions during the biological removal of ammonium from contaminated groundwater in a sequencing batch reactor, where NO3- and NO2- are formed as intermediate products |
| Description: | Analytica Chimica Acta
Volume 841, 2 September 2014, Pages 77-83 Analytica Chimica Acta |
| URI: | http://repository.vnu.edu.vn/handle/VNU_123/30456 |
| ISSN: | 00032670 |
| Appears in Collections: | Bài báo của ĐHQGHN trong Scopus |
Nhận xét
Đăng nhận xét